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目的探讨DD3 mRNA和PSA mRNA在前列腺组织中表达的临床意义及诊断前列腺癌(PCa)的价值。方法荧光定量RT—PCR法分析21例PCa组织、39例良性前列腺增生(BPH)组织DD3 mRNA和PSA mRNA的表达,ROC曲线对DD3 mRNA、PSA mRNA和DD3 mRNA／PSA mRNA比值诊断PCa的价值进行分析。结果PCa组织中DD3 mRNA、PSA mRNA表达量和DD3 mRNA／PSA mRNA比值均显著高于BPH组织,差异有统计学意义(P<0．01)。不同临床分期和分化程度之间DD3 mRNA、PSA mRNA和DD3 mRNA／PSA mRNA比值差异无统计学意义(P值均>0．05)。ROC曲线分析结果显示,DD3 mRNA、PSA mRNA和DD3 mRNA／PSA mRNA的曲线下面积分别为0．937、0．755和0．839。当DD3 mRNA、PSA mRNA和DD3 mRNA／PSA mRNA临界值分别为1．4×105拷贝／mg组织、3．0×107拷贝／mg组织和5．0×10-3时,敏感性分别为90．5％、81．0％和81．0％,特异性分别为85．0％、62．0％和66．7％。若将DD3 mRNA和PSA mRNA联合用于PCa的诊断,其特异性与DD3 mRNA相同,为85．0％,敏感性可达100．0％。结论PCa组织中DD3 mRNA和PSA mRNA表达量均增加,但组织中DD3 mRNA的定量检测更具诊断价值,联合检测有利于提高诊断敏感性,对PCa的诊断具有一定临床应用价值。
Objective To investigate the clinical significance of DD3 mRNA and PSA mRNA expression in prostate tissue and the value of diagnosing prostate cancer (PCa). Methods The expression of DD3 mRNA and PSA mRNA in 21 cases of PCa and 39 cases of benign prostatic hyperplasia (BPH) were detected by real-time fluorescence quantitative RT-PCR. The ROC curve was used to evaluate the diagnostic value of DD3 mRNA, PSA mRNA and DD3 mRNA / PSA mRNA in PCa analysis. Results The ratio of DD3 mRNA, PSA mRNA and DD3 mRNA / PSA mRNA in PCa tissues was significantly higher than that in BPH tissues (P <0.01). There was no significant difference in the ratios of DD3 mRNA, PSA mRNA and DD3 mRNA / PSA mRNA between different clinical stages and differentiation (P> 0.05). ROC curve analysis showed that the area under the curve of DD3 mRNA, PSA mRNA and DD3 mRNA / PSA mRNA were 0.937, 0.755 and 0.839, respectively. When the cutoff values of DD3 mRNA, PSA mRNA and DD3 mRNA / PSA mRNA were 1.4 × 105 copies / mg tissue, 3.0 × 107 copies / mg tissue and 5.0 × 10-3, respectively, the sensitivity was 90 .5%, 81.0% and 81.0% with specificity of 85.0%, 62.0% and 66.7% respectively. If DD3 mRNA and PSA mRNA were used in the diagnosis of PCa, the specificity of DD3 mRNA was 85.0% with a sensitivity of 100.0%. Conclusion The expression of DD3 mRNA and PSA mRNA in PCa tissues are increased, but quantitative detection of DD3 mRNA in tissues is more diagnostic value. Combined detection is helpful to improve the diagnostic sensitivity and has certain clinical value in the diagnosis of PCa.