本文介绍了琼脂糖培养铺一基底单层,用以鉴别研究人周围血中T及B淋巴细胞移动的一种简易法。从6例健康成年供血者各抽取肝素化血10ml(20 IU肝素/ml),无菌加入等量的无Ca~(++)、Mg~(++)的平衡盐溶液(BSS),混匀。取稀释血5ml,小心加于3ml Ficoll-Conray溶液上面,然后按Boyum法,用无菌操作将粒细胞与淋巴细胞分离。将所有淋巴细胞集中在一起,洗涤二次,每次加10ml BSS,在4℃下经450×g离心10分钟后,倒掉上清液,将细胞再悬浮于1 mlBSS中。用Greaves等改良法分离T及B细 This article describes the agarose culture of a basal monolayer to identify human peripheral blood T and B lymphocyte migration of a simple method. Heparinized blood 10 ml (20 IU heparin / ml) was drawn from 6 healthy adult donors, and an equal amount of balanced salt solution (BSS) without Ca ++ and Mg ++ uniform. Take 5ml diluted blood, carefully added to 3ml Ficoll-Conray solution above, and then according to Boyum method, with aseptic operation granulocytes and lymphocytes separated. All lymphocytes were pooled together, washed twice, each time 10 ml of BSS was added, centrifuged at 450 x g for 10 minutes at 4 ° C, the supernatant was discarded and the cells were resuspended in 1 ml of BSS. Use Greaves and other improvement methods to separate T and B fine