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在溶菌酶水溶液中,用NaBH4原位还原HAuCl4得到了溶菌酶稳定的金纳米颗粒。这些溶菌酶稳定的金纳米颗粒具有亲水性、生物兼容性以及良好的溶胶稳定性。紫外-可见光谱、透射电子显微镜以及动态光散射实验对所得产物的表征结果说明实验中形成了分散性良好的直径约2 nm的金纳米颗粒。傅里叶变换红外光谱和圆二色谱实验表明,与金纳米颗粒作用之后的溶菌酶分子仍然保持了结构的完整性,金纳米颗粒的形成没有对蛋白质的一级和二级结构造成影响。基于其良好的溶胶稳定性及生物兼容性,这些溶菌酶稳定的金纳米颗粒在纳米科技和生物医学领域具有潜在的应用价值。
In lysozyme aqueous solution, HAuCl4 was reduced in situ with NaBH4 to obtain lysozyme-stabilized gold nanoparticles. These lysozyme-stabilized gold nanoparticles are hydrophilic, biocompatible, and have good sol stability. UV-Vis spectra, transmission electron microscopy and dynamic light scattering experiments of the resulting product characterization results show that the formation of a good dispersion of gold nanoparticles of about 2 nm in diameter. Fourier transform infrared spectroscopy (FTIR) and circular dichroism (2D-NMR) spectroscopy indicated that the lysozyme molecules remained intact after the interaction with gold nanoparticles. The formation of gold nanoparticles did not affect the primary and secondary structure of the protein. Based on their good sol stability and biocompatibility, these lysozyme-stabilized gold nanoparticles have potential applications in nanotechnology and biomedicine.