论文部分内容阅读
目的为探索角膜移植排斥反应机制，我们对体外异体角膜上皮细胞诱导的外周血Ｔ淋巴细胞亚群ＣＤ６９＋及ＣＤ２５＋的表达进行了测定。方法采用ＣＤ６９＋、ＣＤ２５＋间接免疫荧光标记技术，行流式细胞仪检测及分析ＣＤ６９＋、ＣＤ２５＋的表达率。结果培养的异体角膜上皮细胞与外周血共育３ｈ，测得ＣＤ６９＋表达率较对照组有显著升高（Ｐ＜０．０１）；３ｄ时ＣＤ６９＋表达率稳定维持原有水平（Ｐ＞０．０５）；ＣＤ２５＋的表达则从３ｈ到３ｄ呈现出逐渐上升的结果。结论ＣＤ６９＋是免疫反应过程中早期活化分子，ＣＤ６９＋的表达可能对ＣＤ２５＋的表达起诱导作用
Objective To explore the mechanism of corneal graft rejection, we measured the expression of CD69 + and CD25 + in peripheral blood T lymphocytes induced by allogeneic corneal epithelial cells in vitro. Methods The expression of CD69 + and CD25 + was detected by flow cytometry and CD69 +, CD25 + indirect immunofluorescence staining. Results The cultured corneal epithelial cells were co-cultured with peripheral blood for 3 hours, and the expression of CD69 + was significantly higher than that of the control group (P <0.01). At 3d, the expression of CD69 + was maintained at the original level (P> 0.05 ); CD25 + expression from 3h to 3d showed a gradual increase in the results. Conclusion CD69 + is an early activation molecule in the process of immune response. The expression of CD69 + may play an inducing role on the expression of CD25 +