论文部分内容阅读
目的为探索角膜移植排斥反应机制,我们对体外异体角膜上皮细胞诱导的外周血T淋巴细胞亚群CD69+及CD25+的表达进行了测定。方法采用CD69+、CD25+间接免疫荧光标记技术,行流式细胞仪检测及分析CD69+、CD25+的表达率。结果培养的异体角膜上皮细胞与外周血共育3h,测得CD69+表达率较对照组有显著升高(P<0.01);3d时CD69+表达率稳定维持原有水平(P>0.05);CD25+的表达则从3h到3d呈现出逐渐上升的结果。结论CD69+是免疫反应过程中早期活化分子,CD69+的表达可能对CD25+的表达起诱导作用
Objective To explore the mechanism of corneal graft rejection, we measured the expression of CD69 + and CD25 + in peripheral blood T lymphocytes induced by allogeneic corneal epithelial cells in vitro. Methods The expression of CD69 + and CD25 + was detected by flow cytometry and CD69 +, CD25 + indirect immunofluorescence staining. Results The cultured corneal epithelial cells were co-cultured with peripheral blood for 3 hours, and the expression of CD69 + was significantly higher than that of the control group (P <0.01). At 3d, the expression of CD69 + was maintained at the original level (P> 0.05 ); CD25 + expression from 3h to 3d showed a gradual increase in the results. Conclusion CD69 + is an early activation molecule in the process of immune response. The expression of CD69 + may play an inducing role on the expression of CD25 +