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目的:探讨消癌解毒方体内抗肿瘤作用的可能机制。方法:对荷瘤小鼠(H22肿瘤细胞株)用消癌解毒方煎剂剂量灌胃,观察光镜及电镜下肿瘤组织、细胞的形态,测定基质金属蛋白酶(MMP-2)含量。结果:消癌解毒方中剂量组凋亡细胞数量最大,并且所有消癌解毒方组中凋亡细胞除出现凋亡细胞一般的形态学改变,胞浆中可见大量吞饮空泡;消癌解毒方低剂量组外周血MMP-2水平显著降低(P<0.05),有统计学意义。结论:消癌解毒方对肿瘤抑制作用可能与其诱导肿瘤细胞凋亡,抑制MMP-2活性有关。
Objective: To explore the possible mechanism of antitumor effect of Xiaoyu Jiedu Fang in vivo. Methods: The tumor-bearing mice (H22 tumor cell line) were treated with Xiaojian Jiedu decoction by intragastric administration. The morphological changes of tumor tissues and cells under light and electron microscope were observed. The content of matrix metalloproteinase (MMP-2) was measured. Results: The number of apoptotic cells in the middle-dose group of Xiaoyan Jiedu Decoction was the largest, and all the apoptotic cells in the groups of Xiaoyan Jiedu Decoction except for the general morphological changes of apoptotic cells, a large number of vacuoles could be seen in cytoplasm; The levels of MMP-2 in the low-dose and low-dose groups were significantly decreased (P <0.05), with statistical significance. Conclusion: Xiaojian Jiedu Fang inhibit the tumor may be related to its induction of tumor cell apoptosis, inhibition of MMP-2 activity.