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采用10对EST-SSR引物和9个果实品质性状指标对收集的220份野生刺梨(Rosa roxburghii Tratt)资源的遗传多样性进行分析,结果表明:10对EST-SSR引物共扩增出多态性条带27条,多态百分率为76.67%;9个品质性状中平均单果质量、可滴定酸、固酸比、维生素C和类黄酮含量的变异系数达到20%以上;说明该220份贵州野生刺梨种质资源遗传变异丰富,遗传多样性分布范围较广,适于进行核心种质的构建研究。进一步将9个品质性状指标进行6级分类,并处理为9个品质标记共产生54条多态带,与EST-SSR标记一起,采用Nei’s遗传距离进行UPGMA聚类,同时采用位点优先取样策略进行核心种质构建,以表型保留比例、均值差异百分率、方差差异百分率、极差符合率、变异系数变化率、多态性位点百分率、Nei’s基因多样性指数、Shannon’s信息指数等对核心种质进行评价,并利用主坐标分析法对核心种质进行确认。结果表明:构建的32份核心种质在分子水平,表型水平及地区分布上都能够代表原种质的遗传多样性。
The genetic diversity of 220 Rosa roxburghii Tratt collected from ten pairs of EST-SSR primers and nine fruit quality traits was analyzed. The results showed that 10 pairs of EST-SSR primers amplified polymorphic 27 bands were polymorphic, the percentage of polymorphism was 76.67%. The average single fruit mass, titratable acid-base ratio, vitamin C and flavonoid content in nine quality traits were more than 20% Cili germplasm resources are rich in genetic variation and have a wide range of genetic diversity suitable for the construction of core collection. Further, 9 quality traits were classified into 6 levels and processed for 9 quality markers. A total of 54 polymorphic bands were generated, and together with EST-SSR markers, Nei’s genetic distance was used for UPGMA clustering and site-first sampling strategy The core collection was constructed. The core species, such as phenotypic retention percentage, mean difference percentage, percentage of variance difference, very poor coincidence rate, coefficient of variation coefficient of variation, percentage of polymorphic loci, Nei’s gene diversity index and Shannon’s information index, Qualitative assessment, and the use of the main coordinate analysis of core collections to confirm. The results showed that the 32 core germplasms could represent the genetic diversity of the original germplasm on the molecular level, phenotypic level and geographical distribution.