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目的:建立快速发现车前子吸收成分群的肠外翻模型,为选择车前子质量控制指标提供依据。方法:采用大鼠肠外翻模型,收集3个浓度车前子提取物给药后不同时间的肠囊液样品,用HPLC和LC-MSn进行检测分析,并计算其有效成分京尼平苷酸的累积吸收量。结果:车前子中6种成分均可进入肠囊,在90 min后能全部检测到;不同浓度车前子中京尼平苷酸在各肠段均为线性吸收,R2均大于0.9,符合零级吸收速率;京尼平苷酸在空肠、回肠中的吸收速率常数(Ka)随着车前子提取物浓度增加而增加(P(0.05),符合被动吸收。结论:肠囊对药物成分吸收有选择性,回肠与空肠相比可更多、更快地给出吸收成分信息,选择肠外翻法90 min的样品检测车前子吸收成分比较适宜。
OBJECTIVE: To establish a model of parenchyma for rapid detection of psyllium subelements and provide evidence for the selection of psyllopodia. Methods: The rat intestinal enteral emetic model was used to collect the gut fluid samples of three concentrations of psyllium extract at different time after administration. The gut fluid samples were analyzed by HPLC and LC-MSn. The geniposidic acid The cumulative absorption. Results: All the six components of Psyllium could enter into the gut capsule and could all be detected after 90 min. The concentrations of geniposidic acid in different concentrations of Psyllium were linearly absorbed in all intestine segments, with R2 greater than 0.9 and zero The absorption rate of geniposidic acid in jejunum and ileum (Ka) increased with the increase of plantago (P (0.05)), which accorded with passive absorption.Conclusion: Selective, ileum and jejunum compared to more and more to give information on the absorption component, select the parenteral 90 min samples of Psyllium components more appropriate.