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通过化学分析和酶水解试验,研究了不同的白腐菌对毛白杨的预处理效果及不同组分的降解对酶水解的影响。毛白杨木片经6种白腐菌预处理30d后,各组分都发生了降解,其中半纤维素的损失最为显著,Trametes ochracea C6888引起半纤维素降解率高达47.19%,其次是纤维素和酸不溶木素的降解。在后续酶水解过程中,6种白腐菌处理后的样品显示出不同的水解模式,菌株Trametes ochracea C6888、T.pubescens C7571和T.versicolor C6915预处理效果最为显著,还原糖得率在整个酶水解过程中一直高于对照,其中T.ochraceaC6888在水解96h后还原糖得率达到15.93%,比未处理样品提高了25%。分析酸不溶木素降解率及半纤维素降解率与还原糖得率的关系发现,不同菌株在作用同一种基质时,预处理效果差异显著,木质素和半纤维素的脱除都会影响木质纤维素的酶水解。
Through chemical analysis and enzymatic hydrolysis test, the effect of different white rot fungi on the pretreatment of Populus tomentosa and the degradation of different components on enzymatic hydrolysis were studied. After being pretreated with 6 kinds of white rot fungi for 30 days, the components of Populus tomentosa were degraded, and the loss of hemicellulose was the most significant. Trametes ochracea C6888 caused hemicellulose degradation rate as high as 47.19%, followed by cellulose and acid Insoluble lignin degradation. During the subsequent enzymatic hydrolysis, the six white rot fungi treated samples showed different hydrolysis patterns. The strains Trametes ochracea C6888, T.pubescens C7571 and T. versicolor C6915 had the best pretreatment effect. The hydrolysis rate was always higher than that of the control. Among them, the yield of reducing sugar of T. chracea C6888 reached 15.93% after 96 h of hydrolysis, which was 25% higher than that of untreated samples. Analysis of acid-insoluble lignin degradation rate and hemicellulose degradation rate and reducing sugar yield relationship found that different strains in the same role in the same substrate, the pre-treatment effect is significant differences in lignin and hemicellulose removal will affect the lignocellulosic Enzymatic hydrolysis.