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目的探讨肾母细胞瘤过度表达基因CCN3在宫颈癌的表达及临床意义。方法应用RT-PCR技术检测25例宫颈癌组织及癌旁正常组织CCN3mRNA表达水平,免疫组化法检测43例宫颈癌组织及正常宫颈组织CCN3表达,分析其表达与临床病理特征的关系。结果宫颈癌组织CCN3mRNA表达水平明显高于正常宫颈组织(P<0.05),CCN3在宫颈癌组织中的表达阳性率为58.14%,明显高于正常宫颈组织的6.98%(P<0.05);其表达与病理分级、肿瘤大小及淋巴结转移有显著相关性(P<0.05)。结论 CCN3的表达与宫颈癌的发生、发展有关,可作为宫颈癌诊断及临床监测的分子标志物。
Objective To investigate the expression and clinical significance of nephroblastoma overexpression gene CCN3 in cervical cancer. Methods RT-PCR was used to detect the expression of CCN3 mRNA in 25 cases of cervical cancer and adjacent normal tissues. The expression of CCN3 in 43 cases of cervical cancer and normal cervical tissue was detected by immunohistochemistry. The relationship between CCN3 expression and clinicopathological features was analyzed. Results The positive rate of CCN3 mRNA in cervical cancer was significantly higher than that in normal cervical tissues (P <0.05). The positive rate of CCN3 in cervical cancer was 58.14%, which was significantly higher than that in normal cervical tissues (6.98%, P <0.05) There was a significant correlation between pathological grade, tumor size and lymph node metastasis (P <0.05). Conclusion The expression of CCN3 is related to the occurrence and development of cervical cancer and may be used as a molecular marker for diagnosis and clinical monitoring of cervical cancer.