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目的:研究苇茎Phragmites communis Trin.的化学成分。方法:采用有机溶剂提取, 反复硅胶柱色谱和重结晶法进行分离纯化, 根据化合物的理化性质和波谱数据鉴定其结构; 以A549肿瘤细胞株为研究对象, 采用 MTT 法对所分得的化合物进行体外抗肿瘤活性评价。结果:从苇茎乙醇提取物中分离得到 14 个化合物, 分别鉴定为:金色酰胺醇酯(aurantiamide acetate, 1)、2,3-dihydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-1-propanone (2)、阿魏酸(ferulic acid, 3)、对香豆酸(p-coumaric acid, 4)、丁香酸(syringic acid, 5)、香草酸(vanillic acid, 6)、对羟基苯甲酸(p-hydroxybenzoic acid, 7)、对羟基苯甲醛(p-hydroxybenzaldenhyde, 8)、棕榈酸(palmitic acid, 9)、十七烷酸(heptadecanoic acid, 10)、β-谷甾醇(β-sitosterol, 11)、豆甾醇(stigmasterol, 12)、α-D-葡萄糖(α-D-glucose, 13)和β-D-葡萄糖(β-D-glucose, 14), 它们对A549肿瘤细胞生长无明显抑制活性。结论:化合物1和2为首次从芦苇属植物中分得, 化合物4、5、7、8 和10为首次在该种植物中得到。
Objective: To study the chemical constituents of Phragmites communis Trin. Methods: The compounds were separated and purified by organic solvent extraction, repeated silica gel column chromatography and recrystallization. The structures of the compounds were identified by the physicochemical properties and spectral data of the compounds. The A549 tumor cell lines were used as the research objects, In vitro antitumor activity evaluation. Results: Fourteen compounds were isolated from the ethanolic extracts of reed stems and identified as aurantiamide acetate (1), 2,3-dihydroxy-1- (4-hydroxy-3,5-dimethoxyphenyl) 1-propanone (2), ferulic acid (3), p-coumaric acid (4), syringic acid (5), vanillic acid P-hydroxybenzoic acid (7), p-hydroxybenzaldenhyde (8), palmitic acid (9), heptadecanoic acid (10), β-sitosterol sitosterol, 11), stigmasterol (12), α-D-glucose (13) and β-D-glucose Significantly inhibited activity. Conclusion: Compounds 1 and 2 were isolated from Phragmites for the first time. Compounds 4, 5, 7, 8 and 10 were obtained from this plant for the first time.