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目的：探讨普罗布考（Ｐｒｏｂｕｃｏｌ）对Ｈ_２Ｏ_２诱导的平滑肌细胞（ＳＭＣｓ）增殖的抑制作用。方法：采用细胞计数法和~３Ｈ－ＴｄＲ掺入法观察 ０． １～１００ μｍｏｌ／Ｌ Ｐｒｏｂｕｃｏｌ对新生牛血清（ＮＣＳ）和过氧化氢（Ｈ_２Ｏ_２）促ＳＭＣｓ增殖的抑制作用。结果： １０μｍｏｌ／Ｌ及１００ μｍｏｌ／Ｌ Ｐｒｏｂｕｃｏｌ作用９６ ｈ，可抑制１０％ＮＣＳ刺激的ＳＭＣｓ增殖；而１，１０及１００ μｍｏｌ／Ｌ Ｐｒｏｂｕｃｏｌ作用７２ ｈ及９６ ｈ，均明显抑制 １０μｍｏｌ／Ｌ Ｈ_２Ｏ_２刺激的 ＳＭＣｓ增殖，且呈浓度依赖性（Ｐ＜０． ０５，ｐ＜０． ０１）。 结论： Ｐｒｏｂｕｃｏｌ抑制 ＮＣＳ，Ｈ_２Ｏ_２刺激下体外培养的ＳＭＣｓ增殖，提示其对于动脉粥样硬化和经皮腔内冠状动脉成形术后再狭窄的防治可能具有应用前景。
AIM: To investigate the inhibitory effect of Probucol on H 2 O 2 -induced proliferation of smooth muscle cells (SMCs). Methods: Cell counting method and ~ 3H-TdR incorporation method was used to observe 0. Inhibition of Probucol by 1 ~ 100 μmol / L on Newborn Bovine Serum (NCS) and Hydrogen Peroxide (H_2O_2) - induced SMCs Proliferation. RESULTS: Probucol at 10 and 100 μmol / L for 96 h inhibited the proliferation of 10% NCS-stimulated SMCs. Probucol at 1, 10 and 100 μmol / L for 72 and 96 h inhibited the proliferation of SMMCs induced by 10 μmol / L H_2O_2 Of SMCs proliferated in a concentration-dependent manner (P <0.05, p <0.01). CONCLUSIONS: Probucol inhibits the proliferation of SMCs cultured in vitro stimulated by NCS and H 2 O 2, suggesting that it may have potential applications in the prevention and treatment of atherosclerosis and restenosis after percutaneous transluminal coronary angioplasty.