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目的:明确低氧应激对胶质瘤细胞X-盒结合蛋白1(X-box binding protein 1,XBP1)的作用;明确胶质瘤细胞XBP1表达与糖代谢之间的关系;抑制XBP1表达对胶质瘤细胞在常氧和低氧环境下细胞活力的影响;明确低氧环境中XBP1对胶质瘤细胞糖酵解的影响。方法:分别在常氧和低氧条件下培养人脑胶质瘤细胞系,检测XBP1激活情况;使用si RNA技术抑制XBP1表达,使用氧化磷酸化抑制剂处理细胞,检测细胞活力及糖代谢方式的改变,在常氧和低氧条件下检测细胞存活及糖酵解产物。结果:低氧环境下XBP1活化增加。低氧环境下XBP1沉默降低胶质瘤细胞活力、ATP和乳酸生成,葡萄糖消耗量减少。细胞氧化磷酸化受到抑制后,XBP1沉默降低胶质瘤细胞存活率。结论:低氧环境可诱导胶质瘤细胞XBP1的活化。在低氧环境下XBP1沉默降低胶质瘤细胞活力和糖酵解,胶质瘤细胞的糖酵解依赖于XBP1活化。
OBJECTIVE: To clarify the effect of hypoxia stress on the expression of XBP1 in glioma cells, clarify the relationship between the expression of XBP1 and glucose metabolism, inhibit the expression of XBP1 in glioma cells Glioma cells in normoxia and hypoxia environment, cell viability; clear under hypoxia XBP1 glioma cell glycolytic effects. Methods: The human glioma cell lines were cultured under normoxia and hypoxia respectively to detect the activation of XBP1, the inhibition of XBP1 expression by si RNA technique, the treatment of cells with oxidative phosphorylation inhibitor, the detection of cell viability and glucose metabolism Changed to detect cell survival and glycolysis products under normoxia and hypoxia conditions. Results: XBP1 activation increased under hypoxia. Silencing XBP1 in hypoxia reduced glioma cell viability, ATP and lactate production, and decreased glucose consumption. Silencing of XBP1 reduces glioma cell viability after inhibition of oxidative phosphorylation. Conclusion: Hypoxic environment can induce the activation of XBP1 in glioma cells. Silencing XBP1 in hypoxia reduces the viability and glycolysis of glioma cells, and glycolysis of glioma cells is dependent on XBP1 activation.