论文部分内容阅读
目的:探讨肾癌组织中WNT诱导的分泌型蛋白-1(WISP-1)基因的表达及意义。方法:应用实时RT-PCR和免疫组化法检测31例肾癌组织和18例癌旁正常肾组织中WISP-1mRNA和蛋白的表达。结果:WISP-1mRNA在肾癌组织中的表达中位值(3.37)显著低于癌旁正常组织(9.32),P<0.05;乳头状肾癌(4.67)显著高于透明细胞癌(2.20),P<0.05。WISP-1表达水平与肿瘤分期、大小无显著相关。肾癌组织和正常肾组织中WISP-1免疫组化阳性率分别为71.0%和100%,P<0.05。结论:WISP-1可能与肾癌的发生和分化相关。
Objective: To investigate the expression and significance of WNT-induced secretory protein-1 (WISP-1) gene in renal cell carcinoma. Methods: The expression of WISP-1 mRNA and protein in 31 cases of renal cell carcinoma and 18 cases of adjacent normal renal tissues were detected by real-time RT-PCR and immunohistochemistry. Results: The median expression of WISP-1mRNA in renal cell carcinoma (3.37) was significantly lower than that in adjacent normal tissues (9.32) (P <0.05), and papillary renal carcinoma (4.67) was significantly higher than that in clear cell carcinoma P <0.05. There was no significant correlation between WISP-1 expression and tumor stage and size. The positive rates of WISP-1 immunohistochemistry in renal cell carcinoma and normal renal tissues were 71.0% and 100%, respectively, P <0.05. Conclusion: WISP-1 may be related to the occurrence and differentiation of renal cell carcinoma.