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目的:研究中药红曲对体外培养成骨细胞增殖、分化及矿化功能的影响。方法:取SD大鼠48只,随机将大鼠分为A组(空白组)、B组(倍美力组)、C组(普拉固组)、D、E、F组(红曲高、中、低剂量组)六组,按10 ml/kg分别予以生理盐水、倍美力水溶液(6.25μg/ml)、普拉固水溶液(0.2 mg/ml)及红曲水提液(1.25g/ml、0.625 g/ml、0.125 g/ml)灌胃。灌胃10天后,制备含药血清;将从乳鼠颅骨取材的成骨细胞培养于含药血清培养液中,然后应用MTT法、对硝基苯磷酸盐法及茜素红染色方法观察体外培养成骨细胞、碱性磷酸酶及矿化结节的变化。结果:经过10天红曲水提液灌胃后采血制备的含药血清明显增加了成骨细胞数量(P<0.01或P<0.05)、碱性磷酸酶的表达水平及矿化结节的形成,作用随红曲剂量的增加而增加。结论:成功地运用血清药理学的方法,模拟了体内的药理环境;证实了红曲含药血清可以呈剂量依赖性地促进成骨细胞增殖。
OBJECTIVE: To study the effects of Hongqu, a Chinese medicine, on the proliferation, differentiation and mineralization of osteoblasts cultured in vitro. METHODS: Forty-eight Sprague-Dawley rats were randomly divided into group A (blank group), group B (Bimigli group), group C (Plugu group), group D, E, F (red yeast , medium and low dose groups) six groups, respectively, 10ml/kg physiological saline, premarin solution (6.25μg/ml), prussian solid solution (0.2mg/ml) and red yeast extract (1.25g /ml, 0.625 g/ml, 0.125 g/ml) gavage. 10 days after intragastric administration, drug-containing serum was prepared; osteoblasts derived from the calf’s skull were cultured in medium containing drug serum, and then in vitro culture was observed by MTT method, p-nitrophenyl phosphate method and alizarin red staining method. Changes in osteoblasts, alkaline phosphatase, and mineralized nodules. RESULTS: After 10 days of Hongqu water extracts, the blood serum containing the medicinal herbs significantly increased the number of osteoblasts (P<0.01 or P<0.05), the expression level of alkaline phosphatase and the formation of mineralized nodules. The effect increases with the increase of the dose of red yeast. Conclusion: The serum pharmacology method was successfully used to simulate the pharmacological environment in vivo. It was confirmed that the red yeast drug-containing serum can promote osteoblast proliferation in a dose-dependent manner.