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确定了一株蟹源致病菌(HX-1)为恶臭假单胞菌Pseudomonasputida。该菌对小鼠和河蟹致死率分别为100%(8/8)和87.5%(7/8)。对小鼠的半数致死量LD50为2.9×107/只。同时,该菌还具有凝集鸡、小鼠、牛、鲫、鲢、兔、家鸽的红细胞和粘附Hela细胞的能力。此外,在25℃培养条件下细菌产生的胞外产物(ECP)具有溶血性,但无酪蛋白酶活性;对河蟹有致病性,致死率为60%。
A crab source pathogen (HX-1) was identified as Pseudomonas putida. The mortality of the bacterium to mice and crabs was 100% (8/8) and 87.5% (7/8), respectively. The LD 50 for mice was 2.9 × 10 7 / bird. At the same time, the bacteria also agglutinate chicken, mice, cattle, crucian carp, silver carp, rabbit, domestic pigeon’s red blood cells and the ability to adhere Hela cells. In addition, the extracellular product (ECP) produced by the bacteria at 25 ° C has hemolysis but no caseinase activity and is pathogenic to crabs with a lethal rate of 60%.