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目的分析成人正常肝和肝细胞癌DNA干系倍体及细胞核形态学参数的变化。方法:选取15例成人正常肝和45例肝细胞癌患者的归档蜡块,分别制备4μm、10μm的连续组织学切片,利用TIGER 920G1细胞图像分析仪测量DNA干系倍体值及细胞核形态学参数。结果(1)成人正常肝DNA干系倍体主要以二倍体(73%)为主。(2)肝细胞癌的DNA指数在0.86~9.32之间。45例肝细胞癌病例中DNA干系倍体异质性率为77.8%,其中,34例(75.6%)为DNA干系非整倍体肿瘤,11例(24.4%)为近四/八/十六倍体肿瘤,无1例为DNA干系二倍体肿瘤。结论细胞核DNA干系倍体和细胞核形态学参数分析可作为鉴别人正常肝和肝细胞癌的参考指标。
Objective To analyze the changes of DNA ploidy and nuclear morphological parameters in adult normal liver and hepatocellular carcinoma. Methods: The archived paraffin blocks of 15 normal adult hepatocytes and 45 hepatocellular carcinoma patients were used to prepare serial sections of 4μm and 10μm, respectively. The DNA ploidy and nuclear morphological parameters were measured by TIGER 920G1 image analyzer. Results (1) The normal adult liver DNA haploid mainly diploid (73%) based. (2) The DNA index of hepatocellular carcinoma was between 0.86 and 9.32. Of 45 cases of hepatocellular carcinoma, the rate of DNA haploid heterogeneity was 77.8%, of which 34 (75.6%) were DNA-related aneuploid tumors and 11 (24.4%) were nearly four / eight / 16 Ploidy tumors, none of the DNA-related diploid tumors. Conclusion The analysis of nuclear DNA ploidy and nuclear morphological parameters can be used as a reference index to identify human normal liver and hepatocellular carcinoma.