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目的观察塞来昔布对结肠癌HT-29细胞的生长、凋亡和骨桥蛋白(OPN)表达的影响。方法体外培养结肠癌HT-29细胞分为对照组和不同浓度塞来昔布干预组。MTT法检测细胞增殖抑制情况,流式细胞术分析细胞的凋亡,RT-PCR和免疫组化分析细胞中的OPN mRNA与OPN蛋白表达变化。结果塞来昔布对HT-29细胞增殖有明显的时间与浓度依赖性抑制作用(P<0.01)。塞来昔布能诱导HT-29细胞凋亡,塞来昔布15、30和50μmol/L的细胞凋亡率分别为28.2%、32.8%和33.1%,均明显高于对照组的26.0%(P<0.05)。药物干预组OPN mRNA及OPN蛋白表达明显低于对照组(P<0.01)。结论塞来昔布可能通过抑制OPN表达而抑制结肠癌HT-29细胞的增殖,诱导其凋亡。
Objective To observe the effect of celecoxib on the growth, apoptosis and osteopontin (OPN) expression of colon cancer HT-29 cells. Methods HT-29 colon cancer cells were divided into control group and celecoxib intervention group. The inhibition of cell proliferation was detected by MTT assay. The apoptosis of cells was analyzed by flow cytometry. The expression of OPN mRNA and OPN protein was analyzed by RT-PCR and immunohistochemistry. Results Celecoxib significantly inhibited the proliferation of HT-29 cells in a time-and dose-dependent manner (P <0.01). Celecoxib could induce the apoptosis of HT-29 cells. The apoptosis rates of celecoxib at 15, 30 and 50μmol / L were 28.2%, 32.8% and 33.1%, respectively, which were significantly higher than those in control group (26.0% P <0.05). The expression of OPN mRNA and OPN protein in the drug intervention group was significantly lower than that in the control group (P <0.01). Conclusion Celecoxib inhibits the proliferation and induces apoptosis of HT-29 colon cancer cells by inhibiting OPN expression.