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目前,检测麻疹病毒感染多采用酶联免疫吸附试验(ELISA)检测患者血清中麻疹特异性IgM抗体和采用聚合酶链式反应(PCR)检测麻疹病毒核酸。本文以鞍山市2014年1~3月出现的疑似麻疹病例为样本,分别应用ELISA法检测血清中麻疹特异性IgM抗体和实时荧光PCR法检测麻疹病毒核酸,以探讨两种检测方法检测麻疹病毒的效果及差异性。1临床资料本组标本来源于2014年1~3月鞍山市各区县卫生防疫站和麻疹监测哨点医院送检的疑似麻疹病例共117份,其中血标本于病例出疹后28日内采集、咽拭子标本于病例出疹至
At present, the detection of measles virus infection using enzyme-linked immunosorbent assay (ELISA) detection of measles-specific IgM antibodies in serum and the use of polymerase chain reaction (PCR) detection of measles virus nucleic acid. In this paper, from January to March 2014 in Anshan City, suspected measles cases were taken as samples. ELISA was used to detect measles-specific IgM antibody in serum and real-time fluorescence PCR to detect measles virus nucleic acid. The detection of measles virus by two detection methods Effect and difference. 1 clinical data This group of specimens from January to March 2014 Anshan City District Health and Epidemic Prevention Station and measles surveillance sentinel hospital submitted a total of 117 suspected measles cases, of which blood samples were collected within 28 days after the case rash, pharynx Swab specimens in the case of rash to