穴状昆布 Ecklonia cava Kjellman是一种海洋褐藻。曾报道该植物的乙酸乙酯提取部位对 RNA依赖的DNA聚合酶(RDDP)有较强的抑制作用。作者以生物活性为导向,从穴状昆布中分离出4个均含二苯并[1,4]二氧艺结构的phlorotannins类化合物1～4,研究了它们对HIV-1逆转录酶(RT)和蛋白酶的抑制作用。该植物叶状体干燥后用甲醇提取3次,真空蒸干溶剂,提取物溶于水并依次用正己烷和乙酸乙酯提取。乙酸乙酯部位浓缩后进行硅胶柱层析,用氯仿-乙酸乙酯-甲醇梯度 Cave-like kelp Ecklonia cava Kjellman is a marine brown algae. It has been reported that the ethyl acetate extract of this plant has a strong inhibitory effect on RNA-dependent DNA polymerase (RDDP). The authors isolated four phlorotannins 1-4 containing dibenzo[1,4]dioxygenated structures from cryptands by bioactivity and studied their effects on HIV-1 reverse transcriptase (RT). ) and protease inhibition. The plant fronds were dried and extracted with methanol three times. The solvent was evaporated in vacuo. The extract was dissolved in water and extracted with n-hexane and ethyl acetate in that order. The ethyl acetate fraction was concentrated and subjected to silica gel column chromatography using a chloroform-ethyl acetate-methanol gradient