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Objective:To evaluate the effect of cooling of whole blood samples without an anticoagulant and glucose preservative on glucose and other commonly measured clinical analytes by serial measurements over a three hour period.Methods:Thirty healthy adult volunteers about 20-50 years old,from Kasturba Med ical College,Manipal(2008)were the participants.After obtaining informed consent from each participant,blood was collected(2 mL each)in three vacutainers-one without the use of an anticoagulant and glucose preservative,one with sodium fluoride-potassium oxalate and the other with disodium ethylene diamine tetraacetic(EDTA).Automatic analyzer Hitachi was used to measure glucose and other common clinical laboratory analytes,every hour for three hours.Results:The refrigerated samples without an anticoagulant and preservative,showed slightly lower values of glucose as compared to fluorinated samples but difference between these groups was not statistically significant(P value>0.05).Also cooling had no effect on other analytes except potassium.Conclusion:Fluoride preservative as a regular practice for glucose samples can be avoided if the samples can be processed quickly as fluoriated samples also show a marginal fall in glucose values.