论文部分内容阅读
目的了解佛山庚型肝炎病毒(HGV)感染状况,分析HGV非结构基因(NS)3区部分核苷酸序列。方法采用逆转录聚合酶链反应检测血清HGVRNA,对一例肝炎患者的HGV(HGVC-FS)NS3区818bp片段作克隆及序列分析。结果80例非甲-戊型肝炎患者和105例静脉吸毒者HGVRNA检出率分别为6.3%(5/80)和23.8%(25/105),HGVC-FSNS3区片段核苷酸序列与HGV-U44402、U45966、U36380及HGVC964相同区段同源性为85.5%、85.6%、88.0%、89.2%。结论佛山存在HGV感染,静脉吸毒者感染率较高,HGV可能不是非甲-戊型肝炎主要致病因素。HGVC-FS与HGVC964同源性最高。
Objective To understand the status of hepatitis G virus (HGV) in Foshan and to analyze the nucleotide sequence of the third region of HGV nonstructural gene (NS). Methods Serum HGVRNA was detected by reverse transcription polymerase chain reaction (PCR). The 818 bp fragment of HGV (HGVC-FS) NS3 was cloned and sequenced in a hepatitis patient. Results The detection rates of HGV RNA in 80 patients with non-E-hepatitis E and 105 patients with intravenous drug use were 6.3% (5/80) and 23.8% (25/105), respectively. The HGV-FSNS3 region nucleotide The sequence homology was 85.5%, 85.6%, 88.0% and 89.2% in the same region as HGV-U44402, U45966, U36380 and HGVC964. Conclusion There is HGV infection in Foshan, and the infection rate of intravenous drug users is high. HGV may not be the main causative agent of non-A-hepatitis E infection. HGVC-FS has the highest homology with HGVC964.