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目的:利用代谢组学的方法研究牛血清白蛋白诱导的豚鼠急性过敏反应模型,寻找能够表征该模型的尿液代谢物中的生物标记物。方法:采用基于超高效液相色谱-四极杆飞行时间串联质谱(UPLC-QTof-MS)的代谢组学技术,并结合主成分分析(PCA)和偏最小二乘辨别分析(PLS-DA)的方法,研究正常豚鼠和急性过敏反应组豚鼠尿液代谢物的变化。结果:牛血清白蛋白诱导豚鼠发生强阳性过敏反应。急性过敏反应组与正常对照组豚鼠存在明显的代谢差异,通过查询KEGG数据库确定了4个化合物的结构及代谢途径,包括谷胱甘肽、正辛酸、甲硫基庚基苹果酸和AL-321。结论:代谢组学方法能用于牛血清白蛋白诱导的豚鼠急性过敏反应模型研究,为进一步探索代谢组学在药物急性过敏反应研究中的应用奠定了基础。
OBJECTIVE: To study the acute allergic reaction model induced by bovine serum albumin (BSA) in rats by means of metabonomics and search for biomarkers in urine metabolites that can characterize this model. Methods: Metabolomics based on UPLC-QTof-MS and PCA (PLS-DA) Method to study the normal guinea pigs and acute allergic reaction of urine metabolites of guinea pigs. Results: Bovine serum albumin induced a strong positive allergic reaction in guinea pigs. Acute allergic reaction group and normal control group of guinea pigs there are significant differences in metabolism, by inquiring KEGG database to determine the structure of four compounds and metabolic pathways, including glutathione, n-octanoic acid, methylthio heptyl malic acid and AL-321 . Conclusion: The metabolomics method can be used to study the model of acute allergic reaction induced by bovine serum albumin in guinea pigs, which lays the foundation for further exploring the application of metabolomics in the study of acute allergic reactions.