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目的利用光纤倏逝波生物传感器适合现场快速检测的优势,初步建立1型嗜肺军团菌的快速检测平台。方法优化最佳检测条件,并在此条件下确定检测Lp1的灵敏度和特异性,同时通过检测人工模拟样品确认该方法检测实际样本的灵敏度和特异性。结果该方法 40 min之内自动完成检测。捕获抗体与目标物最佳反应时间、反应体系与检测抗体的最佳反应时间均为10 min,捕获抗体最佳使用浓度是100μg/ml,检测抗体最佳使用浓度为20μg/ml。在此条件下得到该方法检测Lp1的灵敏度为1.8×103cfu/ml,检测的特异性强。结论光纤倏逝波生物传感器快速检测方法为大样本量Lp1污染严重水样的现场快速筛查提供一种新途径。
Objective To establish a rapid detection platform for Legionella pneumophila type 1 by using the advantage of optical fiber evanescent wave bio-sensor for on-site rapid detection. Methods The optimal detection conditions were optimized, and the sensitivity and specificity of Lp1 assay were determined under these conditions. Meanwhile, the sensitivity and specificity of this method to detect real samples were confirmed by testing artificial analog samples. Results The method automatically completed the test within 40 minutes. The optimal reaction time of capture antibody and target was captured. The optimum reaction time of the reaction system and the detection antibody was 10 min. The optimal concentration of the capture antibody was 100 μg / ml and the optimal concentration of the detection antibody was 20 μg / ml. Under this condition, the sensitivity of this method to detect Lp1 was 1.8 × 103 cfu / ml, and the specificity of the assay was strong. Conclusion The rapid detection method of optical fiber evanescent wave biosensor provides a new way for rapid screening of large samples of Lp1 polluted water.