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目的 探讨红细胞膜ＡＴＰ酶（ＡＴＰａｓｅ） 活性变化与糖尿病并发症之间的关系。方法 将９７例糖尿病患者分为三组：Ａ组（无并发症）３７例，Ｂ组（并发酮症酸中毒）１５ 例，Ｃ组（慢性血管并发症）４５例。红细胞膜Ｎａ＋ －Ｋ＋ －ＡＴＰａｓｅ、Ｃａ２＋ －ＡＴＰａｓｅ活性（红细胞膜ＡＴＰａｓｅ活性）分别用改良的Ｈａｎａｈａｎ 法和Ｌｕｔｈｒａ法进行检测，并设４９ 例健康者为对照组。结果 与对照组比较，各组糖尿病患者红细胞膜ＡＴＰａｓｅ活性均明显下降；Ｂ组与Ｃ组的水平相近，但均明显低于Ａ组。在Ｃ组中有心肾功能不全的糖尿病患者红细胞膜两种ＡＴＰａｓｅ 活性最低。结论 红细胞膜ＡＴＰａｓｅ活性下降与糖尿病并发症密切相关，与糖尿病慢性并发症的严重程度相一致的。
Objective To investigate the relationship between changes of erythrocyte membrane ATPase activity and diabetic complications. Methods Ninety-seven patients with diabetes were divided into three groups: group A (without complications), group B (with ketoacidosis) 15, and group C (chronic vascular complications) 45. Erythrocyte membrane Na + -K + -ATPase, Ca2 + -ATPase activity (erythrocyte membrane ATPase activity) were detected by modified Hanahan method and Luthra method, and 49 healthy controls as control group. Results Compared with the control group, the erythrocyte membrane ATPase activity of diabetic patients in each group was significantly decreased; the levels of B and C groups were similar, but both were significantly lower than that of A group. Diabetic patients with cardiorenal dysfunction in group C had the lowest activity of two ATPases. Conclusion The decrease of ATPase activity of erythrocyte membrane is closely related to the complication of diabetes mellitus, which is consistent with the severity of chronic complications of diabetes mellitus.