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目的:研究CD44和p-AKT蛋白在宫颈癌组织中的表达及其与临床病理学的关系。方法:应用免疫组织化学S-P法检测130例宫颈石蜡包埋组织、Western blotting法检测30例宫颈癌及癌旁宫颈组织中CD44和p-AKT蛋白的表达水平。用χ2和t检验分析CD44和p-AKT蛋白在宫颈组织中表达的意义及其与临床病理特征间的关系。结果:免疫组化结果显示在宫颈癌癌变过程中CD44和p-AKT蛋白表达阳性率呈递增趋势(P=0.001,P=0.003);CINⅢ及原位癌组与宫颈癌组CD44和p-AKT蛋白表达阳性率比较差异有统计学意义(P=0.036,P=0.048)。CD44和p-AKT蛋白表达阳性率与宫颈癌患者的年龄无明显相关性,但均与临床分期及组织学分级相关(P(CD44)=0.037,P(CD44)=0.001;P(p-AKT)=0.018,P(p-AKT)=0.002)。p-AKT蛋白的表达与盆腔淋巴结转移有关(P=0.023)。Western Blotting结果显示宫颈癌组织中CD44和p-AKT蛋白表达水平明显高于癌旁组织(P=0.002,P=0.003)。结论:CD44和p-AKT蛋白表达水平在一定程度上可能提示了从CIN向宫颈癌转化的潜能。
Objective: To investigate the expression of CD44 and p-AKT protein in cervical cancer and its relationship with clinicopathology. Methods: The expression of CD44 and p-AKT protein in 30 cases of cervical cancer and adjacent non-cancerous tissues was detected by immunohistochemical S-P method in 130 cases of cervical paraffin embedded tissues. The significance of CD44 and p-AKT protein expression in cervical tissues by χ2 and t-test and their relationship with clinicopathological features were analyzed. Results: Immunohistochemistry showed that the positive rates of CD44 and p-AKT protein in cervical carcinomas were increasing (P = 0.001, P = 0.003). The expressions of CD44 and p-AKT The positive rate of protein expression was significantly different (P = 0.036, P = 0.048). The positive rates of CD44 and p-AKT were not correlated with the age of patients with cervical cancer, but all were correlated with clinical stage and histological grade (P (CD44) = 0.037, P (CD44) ) = 0.018, P (p-AKT) = 0.002). The expression of p-AKT protein was correlated with pelvic lymph node metastasis (P = 0.023). The results of Western Blotting showed that the expression of CD44 and p-AKT in cervical cancer tissues was significantly higher than that in paracancerous tissues (P = 0.002, P = 0.003). Conclusion: The expression of CD44 and p-AKT protein may indicate the potential of transforming from CIN to cervical cancer to a certain extent.