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目的:建立金银花浸膏中绿原酸含量测定方法。方法:采用高效液相色谱法,色谱柱为Kromasil C18色谱柱(4.6 mm×150 mm,5μm);流动相∶乙腈-0.4%磷酸溶液(15∶85);流速:1 mL.min-1;检测波长:327 nm;进样量:10μL。结果:绿原酸在0.106～1.016μg范围内呈良好的线性关系(r=0.999 9),平均回收率为100.3%(RSD=1.04%)。结论:本方法操作简便,准确,重复性好,精密度高,可作为金银花浸膏的质量控制方法。
Objective: To establish a method for determination of chlorogenic acid in honeysuckle extract. Methods: The HPLC method was used. The chromatographic column was Kromasil C18 column (4.6 mm×150 mm, 5 μm). The mobile phase was acetonitrile-0.4% phosphoric acid solution (15∶85). The flow rate was 1 mL.min-1. Detection wavelength: 327 nm; injection volume: 10 μL. RESULTS: The chlorogenic acid showed a good linearity in the range of 0.106-1.016 μg (r=0.999 9). The average recovery was 100.3% (RSD=1.04%). Conclusion: The method is simple, accurate, reproducible, and high precision. It can be used as a quality control method for honeysuckle extract.