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首次从夹竹桃中克隆得到苯丙氨酸解氨酶(PAL)基因cDNA片段,并命名为NoPAL1,GenBank登录号为AY747677,这也是首次从夹竹桃花青苷代谢途径中克隆得到的基因。NoPAL1长866个bp,编码289个氨基酸。通过核苷酸和蛋白质序列多重比较发现NoPAL1与其他植物的PAL基因高度同源。NoPAL1编码的蛋白质序列包含与水稻、玉米PAL蛋白质相同的脱氨基位点和催化活性位点。PAL系统进化树表明,NoPAL1与乔木类植物的PAL基因聚类关系最近。克隆NoPAL1基因为利用基因工程技术来调控夹竹桃花青苷的代谢从而调控夹竹桃花的颜色提供了基因资源。
The cDNA fragment of PAL gene was cloned from oleander for the first time and named NoPAL1. The GenBank accession number was AY747677, which was also the first cloned gene from the oleanolic acid metabolic pathway of oleander. NoPAL1 is 866 bp long and encodes 289 amino acids. NoPAL1 was found to be highly homologous to PAL genes of other plants by multiple comparison of nucleotide and protein sequences. The NoPAL1 encoded protein sequence contains the same deamination sites and catalytically active sites as the rice, maize PAL protein. The PAL phylogenetic tree indicated that NoPAL1 has the closest relationship with the PAL gene cluster of arbors. Cloning NoPAL1 gene provides gene resources for the regulation of the color of oleander flower by using genetic engineering technology to regulate the metabolism of oleanolic acid.