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目的研究HLA-DRB1等位基因与隐匿性乙型肝炎的相关性。方法用PCR-SBT方法对102例隐匿性乙型肝炎患者与201例健康人群对照组进行HLA-DRB1等位基因分型比较并进行相关性分析。结果隐匿性乙型肝炎病例组中HLA-DRB1~*0405(1.980 4%)(χ~2=12.477 0,P=0.000 0,OR=16.367 3)~*0803(4.2573%)(χ~2=30.6898,P=0.000 0,OR=36.454 5)~*1101(5.545 9%)(χ~2=4.471 2,P=0.034 5,OR=1.903 8)~*1201(1.481 6%)(χ~2=8.591 9,P=0.003 4,OR=12.151 5)等位基因频率高于健康对照组(0.124 5%、0.124 5%、3.031%、0.124 5%);OBI病例组中HLA-DRB1~*0701(2.230 8%)(χ~2=5.585 5,P=0.018 1,OR=0.417 7)、~*1301(0.245 4%)(χ~2=4.5137,P=0.0336,OR=0.1474)、~*1302(0.4914%)(χ~2=11.3691,P=0.000 7,OR=0.000 0)、~*1312(0.245 4%)(χ~2=5.019 8,P=0.025 1,OR=0.136 5)、~*1501(4.257 3%)(χ~2=10.763 1,P=0.001 0,OR=0.000 0)等位基因频率分别低于健康对照组(5.105 5%、1.630 2%、3.803 7、1.756 7、9.671 7%)。结论本研究结果显示病例组与健康对照组中HLADRB1~*0701、~*1301、~*1302、~*1312、~*1501有显著性差异(P<0.05,OR<1)可能为OBI的保护基因。HLA-DRB1~*0405、~*0803、~*1101、~*1201可能为OBI的危险基因(P<0.05,OR>1)。
Objective To study the association between HLA-DRB1 alleles and occult hepatitis B (HBV). Methods The HLA-DRB1 alleles were genotyped in 102 cases of occult hepatitis B patients and 201 healthy controls by PCR-SBT. Results In the group of occult hepatitis B, HLA-DRB1 ~ * 0405 (1.980 4%) (χ ~ 2 = 12.477 0, P = 0.000 0, OR = 16.367 3) ~ * 0803 (4.2573% (Χ ~ 2 = 4.471 2, P = 0.034 5, OR = 1.903 8) ~ * 1201 (1.481 6%) (χ ~ 2 = 8.591 9, P = 0.003 4, OR = 12.151 5) The frequency of alleles in HLA-DRB1 ~ * 0701 group was higher than that in healthy control group (0.124 5%, 0.124 5%, 3.031%, 0.124 5% (* 2 = 4.5137, P = 0.0336, OR = 0.1474), ~ * 1301 (0.245 4%) (* 2 = 5.585 5, P = 0.018 1, OR = 0.417 7) (Χ ~ 2 = 5.019 8, P = 0.025 1, OR = 0.136 5) were significantly higher than those in the control group (P = 0.00029, P = 0.0007, OR = 0.000 0) The frequencies of the alleles in the two groups were significantly lower than those in the healthy controls (5.105 5%, 1.630 2%, 3.803 7,1.756 7, P <0.001 0, OR = 0.000 0) , 9.671 7%). Conclusion The results of this study showed that there was a significant difference (P <0.05, OR <1) between HLADRB1 * 0701, ~1301, ~ * 1302, ~ * 1312 and ~ * 1501 in the case group and the healthy control group gene. HLA-DRB1 ~ * 0405, ~ * 0803, ~ * 1101, ~ * 1201 may be risk genes of OBI (P <0.05, OR> 1).