【摘 要】
：
本文采用不同于L?hdetie和Tates的制片方法,通过低渗、固定、软化、离心、分离出精细胞、滴片、吉姆萨染色。比较3种离心方式对制片的影响,发现低于300转/分离心3分钟比较合
【机 构】
：
辽宁省劳动卫生研究所,辽宁省劳动卫生研究所,辽宁省劳动卫生研究所,辽宁省劳动卫生研究所 沈阳 110005,沈阳 110005,沈阳 110005,沈阳 110005
论文部分内容阅读
本文采用不同于L?hdetie和Tates的制片方法,通过低渗、固定、软化、离心、分离出精细胞、滴片、吉姆萨染色。比较3种离心方式对制片的影响,发现低于300转/分离心3分钟比较合适,膜片上细胞均匀分散。杂物较少。不同剂量(40、80、100mg/kg)的环磷酰胺诱发小鼠精细胞微核率不同,剂量越高,微核率也越高。给药后不同处
In this paper, different from L? Hdetie and Tates production methods, through hypotonic, fixed, softened, centrifuged, isolated sperm cells, drop, Giemsa staining. Comparison of three centrifugal methods on the impact of the film and found that less than 300 rev / min centrifugation more appropriate 3 minutes, the diaphragm cells evenly dispersed. Less debris. Different doses (40,80,100mg / kg) cyclophosphamide-induced mouse micronuclei micronucleus rate is different, the higher the dose, the higher the micronucleus rate. Different points after administration
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