论文部分内容阅读
目的：研究不同浓度一氧化氮（ＮＯ）对肺猪泡表面活性物质（ＰＰＳ）替代治疗洗肺鼠疗效的影响。方法：６４只ＳＤ大鼠经反复肺灌洗至ＰａＯ２＜１３．４ｋＰａ和ＰａＣＯ２＞８．０ｋＰａ后，随机分成对照组（组Ⅰ）、ＰＰＳ２５ｍｇ／ｋｇ组（组Ⅱ）、ＰＰＳ５０ｍｇ／ｋｇ组（组Ⅲ）、ＰＰＳ１００ｍｇ／ｋｇ组（组Ⅳ），各组按ＮＯ吸入与否再分成Ａ、Ｂ两亚组，观察用药后ＰａＯ２、ＰａＣＯ２、肺压力一容量环及肺实质密度变化。结果：组ＩＡ的ＰａＯ２、ＰａＣＯ２继续恶化，其中１只大鼠死亡。组ＩＢ在ＮＯ为４０×１０－６Ｍ时ＰａＯ２升高明显。单用ＰＰＳ治疗组其ＰａＯ２升幅及ＰａＣＯ２降幅均以组Ⅳ为最大。ＰＰＳ复合ＮＯ后，组Ⅱ、组Ⅲ的ＰａＯ２均较单用ＰＰＳ组有显著上升，但ＰａＣＯ２的下降不明显。ＮＯ对ＰＰＳ疗效的影响在ＮＯ＝５×１０－６Ｍ时达峰效。各组大鼠的肺ＰＶ环及肺实质密度以组Ⅳ为最好，随后依次为组Ⅲ和组Ⅱ，对照组最差。结论：吸入ＮＯ后可加强ＰＰＳ改善氧合的功能，减少ＰＰＳ用量。吸入ＮＯ５×１０－６即能明显改善ＰＰＳ的疗效
Objective: To study the effects of different concentrations of nitric oxide (NO) on the efficacy of pulmonary surfactant (PPS) replacement therapy in mice. Methods: Sixty-four Sprague-Dawley rats were randomly divided into control group (group Ⅰ), PPS25mg / kg group (group Ⅱ), PPS50mg / kg group (group A) after repeated lung lavage to PaO2 <13.4kPa and PaCO2> 8.0kPa Ⅲ), PPS100mg / kg group (group Ⅳ) .All groups were subdivided into A and B subgroups according to NO inhalation. The change of PaO2, PaCO2, pulmonary pressure-volume ring and lung parenchymal density were observed. Results: PaO2 and PaCO2 in Group IA continued to deteriorate, of which 1 died. PaO2 significantly increased when NO was 40 × 10-6M. In the PPS group, the increase of PaO2 and the decrease of PaCO2 in group P were the highest in group Ⅳ. After PPS combined with NO, PaO2 in group Ⅱ and group Ⅲ increased significantly compared with that in group PPS alone, but the decrease of PaCO2 was not obvious. The effect of NO on the efficacy of PPS peaked at NO = 5 × 10-6M. The rats in each group of pulmonary P V ring and lung parenchyma density to group Ⅳ is the best, followed by the group Ⅲ and group Ⅱ, the worst control group. Conclusion: NO inhalation can enhance the PPS to improve oxygenation function, reduce the amount of PPS. Inhalation NO5 × 10-6 that can significantly improve the efficacy of PPS