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应用大麻哈鱼(Oncorhynchus keta)促性腺激素(GTH)诱发大西洋鲑(Salmosalar)卵黄发生期的离体卵巢滤泡(直径为3.4毫米)产生性类固醇激素的研究表明,不论用0.1或1微克/毫升GTH刺激,经培育10天后,未出现卵母细胞卵核消失的现象,卵核偏位也不明显。对培养液中17α,20β-双羟孕酮(17α20βP)的积累浓度测定结果,仅1微克/毫升GTH组含少量的17α20βP;0.1微克/毫升GTH组,培养液中雌二醇的积累浓度明显高于对照组和1微克/毫升GTH组,并为后者的两倍。除雌二醇外,孕酮,17α-羟孕酮,17α20βP,雄烯二酮和睾酮的产生,都呈现对GTH剂量的依赖关系,1微克/毫升GTH组的产生量明显高于0.1微克/毫升GTH组和对照组。离体研究的结果表明,处于卵黄发生期的卵巢滤泡对低剂量(0.1微克/毫升)和高剂量(1微克/毫升)GTH的反应不同。低浓度的GTH,引起性类固醇激素生物合成的途径沿着形成雌二醇的方向进行,雄激素被芳香化成雌激素,雌二醇再而刺激肝脏合成卵黄物质的前体,从而促进卵黄发生。高浓度的GTH,有抑制雌二醇合成的作用,而是促使卵母细胞向着最终成熟的方向发展,使住类固醇生物合成的途径转向形成大量雄激素和孕激素,特别是17α20βP。这与已充分生长和卵黄积累完成的卵巢滤泡离体试验的结果一致,与活体观察以上各种性类固醇激
Studies on the production of sex steroid hormones using ovariectomized follicles (3.4 mm in diameter) in the salmonella salmonella stage induced by Oncorhynchus keta gonadotropin (GTH) showed that either 0.1 or 1 μg / Ml GTH stimulation, after 10 days of incubation, there was no oocyte oocyte disappear phenomenon, ovarian deviation is not obvious. The concentration of 17α, 20β-progesterone (17α20βP) in the culture fluid was measured. Only 1 μg / ml GTH group contained a small amount of 17α20βP and 0.1 μg / ml GTH group. The concentration of estradiol in the culture fluid was significantly increased Higher than the control group and the 1 microgram / ml GTH group, and double the latter. In addition to estradiol, the progesterone, 17α-hydroxyprogesterone, 17α20βP, androstenedione and testosterone production showed a dose-dependent GTH, 1 μg / ml GTH group was significantly higher than the production of 0.1 μg / Ml GTH group and control group. The results of the in vitro study showed that ovarian follicles at the stage of yolk production responded differently to low doses (0.1 μg / ml) and high doses (1 μg / ml) of GTH. Low concentrations of GTH, causing sex steroid hormone biosynthesis pathway along the direction of the formation of estradiol, androgens are aromatized to estrogen, estradiol and then stimulate the liver to synthesize the precursor of yolk material, thereby promoting the occurrence of yolk. High concentrations of GTH, which inhibit the synthesis of estradiol, prompt the oocyte toward the eventual maturation, diverting the steroid biosynthetic pathway to the formation of large quantities of androgens and progestins, especially 17α20βP. This is consistent with the in vitro test of ovarian follicles that have been well-established and yolk accumulation has been completed, as compared with in vivo observations of various sex steroid stimulations