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本文的目的在于观察铜离子对草履虫的繁殖毒性、有丝分裂相细胞比率、生长抑制和刺丝泡不发射率的作用。结果表明：Ｃｕ２＋对草履虫产生繁殖毒性，有丝分裂指数降低，生长抑制的阈浓度为０．０１９５ｍｇ／Ｌ（Ｐ＜０．０１）。随Ｃｕ２＋浓度的逐渐增加，相对繁殖率逐渐降低，刺丝泡的不发射率逐渐增高，存在明显的浓度－反应关系（Ｐ＜０．０１）。但是，分裂相细胞比率的下降和体长的阶梯性抑制均无明显浓度依赖关系（Ｐ＞０．０５）。我们的结果表明长时间接触浓度大于０．０１９５ｍｇ／Ｌ的Ｃｕ２＋对草履虫的ＤＮＡ有损伤作用，干扰蛋白质、核酸等生物大分子的合成，从而影响草履虫的细胞分裂、繁殖和生长。本研究提出繁殖实验是监测铜离子对水生物慢性毒性敏感的方法。
The purpose of this paper is to observe the effects of copper ions on reproductive toxicity, mitotic cell ratio, growth inhibition and non-eruption rate of Paramecium. The results showed that Cu2 + had reproductive toxicity and mitotic index decreased, and the threshold concentration of growth inhibition was 0.0195 mg / L (P <0.01). With the gradual increase of Cu2 + concentration, the relative reproductive rate decreased gradually, and the non-emissivity of barnospore increased gradually. There was a significant concentration-response relationship (P <0.01). However, there was no significant concentration-dependent relationship between the ratio of split-phase cells and the step-down inhibition of body length (P> 0.05). Our results show that prolonged exposure to Cu2 + at a concentration greater than 0.0195 mg / L may impair the DNA of Paramecium, interfere with the synthesis of biological macromolecules such as proteins and nucleic acids, and thus affect the division, reproduction and growth of Paramecium. In this study, breeding experiments were proposed to monitor the sensitivity of copper ions to the chronic toxicity of aquatic organisms.