论文部分内容阅读
目的 观察通脉益智胶囊对脑缺血再灌注损伤诱导的小鼠海马神经细胞凋亡的影响 ,探讨其神经保护作用的机制。方法 雄性ICR小鼠 4 0只 ,随机分为 4组 :假手术组、模型组、通脉益智胶囊组、西药 (喜德镇 )对照组。建立小鼠反复脑缺血再灌注模型。分别采用光学显微镜、透射电子显微镜观察 ,原位末端标记法 (TUNEL)染色并计算凋亡指数 (ApoptosisIndexAI)。结果 通脉益智胶囊组病理损害明显减轻 ,凋亡指数显著低于模型组及西药对照组。结论 通脉益智胶囊可显著抑制由脑缺血再灌注损伤诱导的小鼠海马神经细胞凋亡 ,从而起到一定程度的神经保护作用
Objective To observe the effect of Tongmai Yizhi Capsule on the apoptosis of hippocampal neurons induced by cerebral ischemia-reperfusion injury in mice, and to explore the mechanism of neuroprotective effect. Methods Forty male ICR mice were randomly divided into 4 groups: sham operation group, model group, Tongmai Yizhi capsule group, and Western medicine (Xidedezhen) control group. A mouse model of repeated cerebral ischemia-reperfusion was established. Using light microscopy and transmission electron microscopy, respectively, TUNEL staining was performed and the apoptosis index (Apoptosis IndexAI) was calculated. Results The pathological damage of Tongmai Yizhi capsule group was significantly reduced, and the apoptotic index was significantly lower than that of the model group and the western medicine control group. Conclusion Tongmai Yizhi capsule can significantly inhibit the apoptosis of hippocampal neurons in mice induced by cerebral ischemia-reperfusion injury, and thus play a role in neuroprotection.