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目的建立小鼠严重颅脑创伤模型探讨粒细胞集落刺激因子(granulocyte colony-stimulating factor,G-CSF)动员循环间充质干细胞(mesenchym alstem cells,MSCs)的可行性,并观察G-CSF动员的修复效应。方法以G-CSF作为动员剂,培养骨髓和外周血来源的MSCs,计数动员前后的CFU-F。制作小鼠严重颅脑创伤模型,致伤后采用G-CSF对MSCs进行动员,在2、24、48、96、120、144、192、264、336h进行神经行为学评分、运动功能评分,并观察小鼠死亡率和病理组织切片。结果从小鼠外周血培养出MSCs,G-CSF动员后骨髓和外周血MSCs的CFU-F数量显著高于正常对照组(P<0·01)。致伤后小鼠神经行为学和运动功能评分显著低于正常对照组(P<0·01),创伤治疗组在144～192h期间评分显著高于创伤组(P<0·05)。致伤后小鼠死亡率增高,病理切片可见创伤处有出血和空泡。结论成功培养及用G-CSF动员了外周血MSCs,在成功建立小鼠严重颅脑创伤模型的基础上,显示G-CSF动员可以降低严重颅脑创伤小鼠死亡,参与了创伤修复。
Objective To establish a model of severe traumatic brain injury in mice and investigate the feasibility of granulocyte colony-stimulating factor (G-CSF) mobilizing mesenchymal stem cells (MSCs) Repair effect. Methods MSCs derived from bone marrow and peripheral blood were cultured with G-CSF as a mobilizer, and CFU-F before and after mobilization was counted. The model of severe traumatic brain injury in mice was made. MSCs were mobilized with G-CSF after injury, and neurobehavioral scores, motor function scores at 2, 24, 48, 96, 120, 144, 192, 264, Mortality and histological sections were observed. Results MSCs were cultured from mouse peripheral blood. The numbers of CFU-F in bone marrow and peripheral blood MSCs after G-CSF mobilization were significantly higher than those in normal control group (P <0.01). The score of neurobehavioral and motor function in mice after wounding was significantly lower than that in normal control group (P <0.01). The score of wound healing group in 144 ~ 192h was significantly higher than that in trauma group (P <0.05). After the injury, the mortality rate of mice was increased. Pathological examination revealed bleeding and vacuolization at the wound site. Conclusion Successful mobilization and mobilization of peripheral blood MSCs with G-CSF have established a successful model of severe traumatic brain injury in mice and showed that G-CSF mobilization can reduce the death of severe traumatic brain injury and participate in wound repair.