The Yong’an-Meitai area is the focus of the present exploration in the Fushan Depression,Beibuwan Basin,South China Sea. All oils from this area are geochemically characterized by higher Pr/Ph ratio,higher proportion of heavy molecular weight hydrocarbons,and higher proportion of C29 regular steranes,which indicate that the organic matter of source rocks might have been deposited in an oxidizing palaeoenvironment and be dominated by higher plant organic matter input. The oil from E3w2 (the second member of Weizhou Fm. of the Oligocene) has a much higher density,relatively higher Pr/nC17 and Ph/nC18 ratios,and a “UCM-unresolved complex mixture” on gas chromatograms,which indicate that it has been slightly biodegraded. CPI and other terpane and sterane isomer ratios suggest they are all mature oils. The timing of oil charging in E3w2 and E2l1 (the first member of the Liushagang Fm. of the Eocene) determined by the homogenization temperatures of fluid inclusions and thermal evolution history are from 9-3 Ma and 8-3 Ma,respectively. Thus,the interpretation of E3w2 as a secondary reservoir is unlikely. The timing of oil charging is later than that of hydrocarbon generating and expulsion of Liushagang Fm. source rocks and trap formation,which is favorable for oil accumulation in this area. All molecular parameters that are used for tracing oil filling direction decrease with shallower burial depth,which suggests vertical oil migration. The widely occurring faults that penetrate through the source rocks of the Liushagang Fm. may serve as a fine oil charging conduit. The Yong’an-Meitai area is the focus of the present exploration in the Fushan Depression, Beibuwan Basin, South China Sea. All oils from this area are geochemically characterized by higher Pr / Ph ratios, higher proportion of heavy molecular weight hydrocarbons, and higher proportion of C29 regular steranes, which indicates that the organic matter of source rocks might have been deposited in an oxidizing paeoenvironment and be dominated by higher plants organic matter input. The oil from E3w2 (the second member of Weizhou Fm. of the Oligocene) has a much higher density, more higher Pr / nC17 and Ph / nC18 ratios, and a “UCM-unresolved complex mixture ” on gas chromatograms, which indicates that it has been slightly biodegraded. CPI and other terpane and sterane isomer ratios suggest suggest The timing of oil charging in E3w2 and E2l1 (the first member of the Liushagang Fm. of the Eocene) determined by the homogenization temperatures of fluid inclusions and thermal evolution hi Thus, the interpretation of E3w2 as a secondary reservoir is unlikely. The timing of oil charging is later than that of hydrocarbon generating and expulsion of Liushagang Fm. source rocks and traps formation, which is favorable for oil accumulation in this area. All widely used faults for penetrate through the source rocks of the Liushagang Fm. may serve as a fine oil charging conduit.