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稻瘟病是我国水稻的主要病害。由于对病原菌致病性变异的机制缺乏了解,许多旨在培育稳定抗性品种的育种计划未能收到良好效果。因此对病原菌的遗传多样性进行研究,有助于明确新致病型产生的机理;以及病原菌群体遗传结构变化与病害流行的关系。本研究利用美国Purdue大学提供的一个散布的重复序列探针MGR-586和限制性内切酶EcoRI,对我国南方部分地区稻瘟病菌DNA的限制性片段长度多态性进行了研究。
Rice blast is the main disease of rice in our country. Due to a lack of understanding of the mechanisms of pathogenic variation in pathogens, many breeding programs aimed at cultivating stable resistant varieties failed to achieve good results. Therefore, the study of genetic diversity of pathogenic bacteria will help clarify the mechanism of the new pathogenicity; and the relationship between the genetic structure of the pathogen population and the epidemic. In this study, the restriction fragment length polymorphism of Magnaporthe grisea DNA in some areas of southern China was studied by using a scattered repeat probe MGR-586 and restriction endonuclease EcoRI provided by Purdue University in the United States.