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用Operon公司OPA - 0 1～OPJ- 2 0 2 0 0个 1 0碱基对随机序列引物对甘蓝型油菜隐性核不育涪优AB ,显性核不育 37AB ,广恢系 98- 80 2 6 ,98- 3749及F1材料基因组DNA进行RAPD扩增的结果 ,共获得 1 334条扩增带 ,平均每引物扩增带约 6 .7条 ,表明油菜存在较为丰富的遗传多样性。引物OPA - 0 5 ,OPA - 0 9,OPA - 1 2 ,OPA - 1 3,OPA - 2 0等分别扩增出了大小不同的多态性带 ,初步认为OPA - 1 2 950 是 37A ,OPA - 2 0 750 是涪优A的不育基因分子标记 ,OPA - 0 5 150 0 ,OPA - 1 33 550 是与育性基因有关的带 ,OPA - 1 32 3 0 0 是甘蓝型油菜材料的一条特征带。
Operon OPA-0 1 ~ OPJ-220 0 1 0 base pairs of random sequence primers were used in Brassica napus recessive nuclear fertility Fuyou AB, dominant nuclear sterility 37AB, Guang Hui line 98- 80 A total of 1 334 amplified bands were obtained by RAPD amplification of genomic DNA from 26, 98- 3749 and F1. The average amplified bands per primer were about 6.7, indicating that Rape has a rich genetic diversity. Primers OPA - 0 5, OPA - 0 9, OPA - 1 2, OPA - 1 3 and OPA - 2 0 amplified polymorphic bands of different sizes, and initially considered OPA - 1 2 950 as 37A, OPA - 2 0 750 is a molecular marker of male sterile gene Fuyao A, OPA - 0 5 150 0 and OPA - 1 33 550 are bands related to fertility genes. OPA - 1 32 30 is a Feature band.