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目的分析龙岩市2011年病毒性脑炎病例埃可病毒[人肠道致细胞病变孤儿病毒,Enteric Cytopathogenic Human Orphan(ECHO)Virus]30型(ECHO30型)特征。方法对龙岩市2011年某医院病毒性脑炎住院病例采集脑脊液(Cerebrospinal Fluid,CSF)进行病毒分离、血清中和试验定型;选取4株ECHO30型分离株,测定VP1编码区序列,与国内外其他病毒株进行比较。结果从142份患者CSF标本中分离到ECHO30型20株、ECHO11型7株、ECHO6型10株。4株ECHO30型VP1编码区序列长度为606个核苷酸,编码202个氨基酸。4株的核苷酸和氨基酸同源性分别为97%~100%和88%~100%。与2000年Bastianni原型株的核苷酸和氨基酸同源性分别为81%~82%和81%~93%。与2010年山东省、2011和2012年福州市流行毒株核苷酸和氨基酸同源性分别为96%~100%和87%~100%。系统进化树分析显示,4株龙岩分离株同属基因型(Genotype)Ⅱ。结论龙岩市2011年病毒性脑炎病例的ECHO_(30)型为GenotypeⅡ。
Objective To analyze the characteristics of echovirus (Enteric Cytopathogenic Human Orphan (ECHO) Virus) 30 (ECHO30) in patients with viral encephalitis in Longyan in 2011. Methods Cerebrospinal fluid (CSF) collected from hospital in a hospital of Longyan City in 2011 was subjected to virus isolation and serum neutralization test. Four ECHO30 isolates were selected and sequenced to determine the coding region of VP1. Virus strains were compared. Results ECHO30 type 20 strains, ECHO11 type 7 strains and ECHO6 type 10 strains were isolated from CSF samples of 142 patients. Four ECHO30 type VP1 coding region length of 606 nucleotides, encoding 202 amino acids. The nucleotide and amino acid homology of four isolates were 97% ~ 100% and 88% ~ 100%, respectively. Nucleotide and amino acid homology with the Bastianni prototype strain in 2000 was 81% -82% and 81% -93%, respectively. Compared with Shandong Province in 2010, the nucleotide and amino acid homology of the strains in Fuzhou city in 2011 and 2012 were 96% -100% and 87% -100%, respectively. Phylogenetic analysis showed that four isolates from Longyan belonged to genotype Ⅱ. Conclusion ECHO (30) genotypes of cases of viral encephalitis in Longyan in 2011 are Genotype Ⅱ.