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将高粱总DNA通过花粉管通道导入小麦感病品种甘麦8号,D2代出现2株对条锈病免疫的变异株,D5代有9个株系抗性已经稳定;用混合菌和分小种鉴定,对条中29、30、洛13Ⅱ、水14、水14中梁17-s、HY3、条中31号等小种表现免疫。结果分析表明,新品系89144接种后组织中SOD活性升高,原受体甘麦8号接种锈菌后SA含量也有升高;但并不伴随有CAT活性下降,SOD活性和H2O2含量的升高;推测SA为CAT过氧化活性提供一个电子的过程中SA含量必须达到一定的阈值,并且与CAT的时序调节相配合;据SA结合态和游离态含量的变化动态,表明89144具有SA信号传导途径,推测SA信号传导途径的上游应该还有一些机制在起作用。
The total DNA of sorghum was introduced into the wheat susceptible cultivar “Ganmai 8” via pollen tube channel. Two mutants were found to be infected with the stripe rust in D2 generation. The resistance of 9 strains in D5 was stable. Identification, the article 29,30, Luo 13 Ⅱ, water 14, water 14 Zhong Liang 17-s, HY3, No. 31 and other races showed immunity. The results showed that the SOD activity of the new line 89144 inoculated was increased, and the content of SA was also increased after the original recipient Ganmai 8 inoculated with the rust, but not accompanied by the decrease of CAT activity, the increase of SOD activity and the content of H2O2 ; It is speculated that the SA content in the process of providing an electron for the CAT peroxidation activity must reach a certain threshold value and be coordinated with the timing regulation of CAT. According to the dynamic change of SA bound content and free content, it is suggested that 89144 has the SA signal transduction pathway, It is speculated that there may be some mechanisms upstream of the SA signaling pathway.