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目的了解深圳市活禽市场外环境中禽流感病毒的分布情况,为进一步探索和预测人禽流感变化规律提供参考。方法在全市10个区分别设立一个采样点,采集相应的外环境拭子标本,采用荧光定量PCR方法检测甲型流感病毒(Flu A),以及H5和H9亚型病毒核酸。结果 2015年12月至2016年5月共采集外环境标本1 204份。Flu A的核酸检测阳性率为66.7%,H5亚型阳性率为30.2%,H9亚型阳性率为33.1%,H5和H9亚型检测均为阳性的,阳性率为15.9%。H5与H9亚型差异无统计学意义(χ2=2.219,P>0.05)。结论深圳市活禽市场外环境存在禽流感病毒污染,H5亚型与H9亚型同时存在,且阳性率均在30%以上,有明显的混合感染,表明二者之间存在着发生重配的可能。
Objective To understand the distribution of avian influenza virus in live environment of live poultry market in Shenzhen and to provide reference for further exploration and prediction of the change rule of human bird flu. Methods A sampling site was set up in 10 districts of the city to collect the corresponding swab samples from the external environment. Flu A and H5 and H9 subtype virus nucleic acids were detected by fluorescence quantitative PCR. Results A total of 1 204 samples of environmental samples were collected from December 2015 to May 2016. The positive rate of nucleic acid detection of Flu A was 66.7%, the positive rate of H5 subtype was 30.2%, the positive rate of H9 subtype was 33.1%, and the positive rate of H5 and H9 was 15.9%. There was no significant difference between H5 and H9 subtypes (χ2 = 2.219, P> 0.05). Conclusions The live poultry market outside Shenzhen has bird flu virus contamination. H5 subtypes and H9 subtypes are present simultaneously with a positive rate of more than 30%, indicating a significant mixed infection, indicating that there is a reassortment between the two may.