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应用rhIL 18在体外培养系统 (coculturesysteminvitro ,CCS )中诱导肿瘤特异性细胞毒性T淋巴细胞 (cytotoxicTlympho cyte ,CTL ) ,探索不同细胞在IL 18起动和促进肿瘤特异性免疫应答方面的作用。采用StemSepTM免疫磁性细胞分离法分离人外周血NK细胞、T细胞及树突细胞 (DC ) ,流式细胞仪分析细胞表型 ,12 5I UdR标记的细胞毒实验检测杀伤活性。结果表明 ,在肿瘤抗原存在的条件下 ,CCS中rhIL 18能够诱导并促进CTL介导的肿瘤特异性杀伤效应 ;并且 ,在rhIL 18诱导肿瘤特异性CTL的过程中 ,rhIL 18、NK细胞、DC及T细胞均起着十分重要的作用 ,缺少任一组份 ,均对诱导肿瘤特异性CTL有明显差异 (P <0 0 1)。提示在CCS中 ,rhIL 18诱导的NK细胞快速杀伤效应及DC的抗原提呈作用 ,在肿瘤特异性CTL产生过程中 ,均起着决定性的作用。
The use of rhIL 18 induces tumor-specific cytotoxic T lymphocyte (CTL) in an in vitro culture system (coculturesysteminvitro, CCS) to explore the role of different cells in IL 18 priming and in promoting tumor-specific immune responses. Human peripheral blood NK cells, T cells and dendritic cells (DCs) were isolated by StemSepTM immunomagnetic separation. Cell phenotypes were analyzed by flow cytometry. Cytotoxicity was detected by 125I UdR cytotoxicity assay. The results showed that under the condition of tumor antigen, rhIL 18 could induce and promote CTL-mediated tumor-specific killing effect in CCS. Moreover, in the process of inducing tumor-specific CTL by rhIL 18, rhIL 18, NK cells, DC And T cells play a very important role in the absence of any component, were induced tumor-specific CTL were significantly different (P lt; 0 01). It is suggested that the rapid killing effect of NK cells induced by rhIL 18 and the antigen presenting effect of DC in CCS play a decisive role in the tumor-specific CTL production.