论文部分内容阅读
目的调查宫颈部人乳头瘤病毒(HPV)高危型感染情况及其基因亚型分析,为HPV感染分子流行病学研究及宫颈癌防治提供依据。方法采用凯普导流杂交HPV DNA检测法,对疑似HPV感染的1 585例宫颈脱落细胞进行21种HPV亚型检测;并对高危型HPV阳性患者进行薄层液基细胞学检查(TCT)和病理活检。结果 1 585例标本中有414例HPV检测阳性,感染率为26.12%,其中高危型占82.61%,单一高危亚型感染占83.33%,其主要基因亚型是52,16和38,检出频率分别26.57%,25.60%和13.29%;HPV高危型感染率随着年龄的增长而升高,<21岁除外;342例高危型HPV阳性患者中有38例液基薄层细胞学检查阳性,33例病理活检阳性。结论 1 585例中宫颈部人乳头瘤病毒高危型感染较严重,以单一高危亚型感染为主,HPV52,16和38是其中的主要高危亚型。
Objective To investigate the status and genotypic analysis of high risk HPV infection in cervical region and provide basis for molecular epidemiology and prevention and treatment of cervical cancer. Methods Twenty-one HPV subtypes were detected in 1 585 cervical exfoliated cells with HPV infection by using the HPV DNA detection method. The high-risk HPV-positive patients were examined by thin-layer liquid-based cytology (TCT) and Biopsy. Results Of the 1 585 specimens, 414 were HPV positive, with an infection rate of 26.12%, of which 82.61% were high risk type, 83.33% were single high risk subtype, and the major gene subtypes were 52, 16 and 38. The detection frequency 26.57%, 25.60% and 13.29% respectively. The infection rate of high-risk HPV increased with age except <21 years old. Thirty-eight of the 342 high-risk HPV-positive patients had liquid-based thin- Case biopsy positive. Conclusion 1 585 cases of cervical HPV infection are more likely to be at high risk, with a single high-risk subtype of infection. HPV52, HPV16 and HPV38 are the major high risk subtypes.