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目的:评估丙二醛变性高效液相色谱法在胚胎移植过程中的临床价值。方法:收集我院生殖中心2010年2月~2012年3间未做移植的废弃胚胎,由D2胚胎培养到D3胚胎期,提取遗传物质并进行PRC扩增,采用高形态学评分的D3作为对照,高效液相色谱法测定遗传DNA过氧化产物丙二醛(MDA)的含量。结果:多核Ⅰ组D2期卵裂球核≥2个,共24枚胚胎,DNA过氧化产物丙二醛(MDA)的含量5.32±0.19μmol/L,对比正常胚胎0.67±0.08μmol/l有显著差异(P<0.05)。多核Ⅱ组D2期卵裂球核=2,共19枚胚胎,MDA的含量4.12±0.22μmol/L,对比正常胚胎0.67±0.08μmol/l有显著差异(P<0.05)。Ⅰ组与Ⅱ组间没有统计学差异。然而,空泡胚胎与非空泡胚胎之间MDA并没有差异。结论:多核D2期胚胎培养到D3期会产生很高异常率,因此临床上应减少使用此种进行移植。
Objective: To evaluate the clinical value of malondialdehyde-denatured high performance liquid chromatography in embryo transfer. Methods: Three non-transplanted embryos were collected from February 2010 to March 2012 in our reproductive center from D2 embryo to D3 embryo stage. Genetic material was extracted and amplified by PCR. D3, a high morphological score, was used as a control The contents of malondialdehyde (MDA), a product of genetic DNA, were determined by HPLC. Results: There were 24 embryos at stage D2 in D2 group, and the content of MDA was 5.32 ± 0.19μmol / L compared with 0.67 ± 0.08μmol / L in normal embryos Difference (P <0.05). There were 19 embryos in D2 stage of multinucleated group Ⅱ, the content of MDA was 4.12 ± 0.22μmol / L, which was significantly different from that of normal embryos (0.67 ± 0.08μmol / L) (P <0.05). There was no significant difference between group Ⅰ and group Ⅱ. However, MDA did not differ between vacuolar and non-vacuolar embryos. CONCLUSION: Multicore D2 embryos cultured to D3 have a very high rate of abnormalities and should therefore be used less clinically for transplantation.