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目的探讨发热伴粒细胞血小板减少综合征布尼亚病毒(Severe fever with thrombocytopenia syndrome bunya-virus,SFTSV)在细胞分离培养时表现的特异性。方法收集发病一周以内发热伴粒细胞血小板减少综合征患者的血液,首先做血清学检测排除汉坦病毒(Hantavirus,HV),然后将样本采用多种细胞培养的方法进行病毒分离。最后采用逆转录(RT-PCR)方法对病毒进行鉴定。结果从我省发热伴出血征候群血液样本分离出病毒12株,经鉴定均为新布尼亚病毒。结论我省东部山区及丘陵地带有新布尼亚病毒流行,其临床症状及体征与肾综合征出血热极为相似。但体外细胞培养及核苷酸序列却存在着明显的差别。
Objective To investigate the specificity of fever and thrombocytopenia syndrome bunya-virus (SFTSV) in cell isolation and culture. Methods Blood was collected from patients with fever and granulocytopenia syndrome within one week of onset. Hantavirus (HV) was first detected by serological tests and then the virus was isolated by a variety of cell culture methods. Finally, the virus was identified by reverse transcription (RT-PCR). Results From our province fever with bleeding syndrome blood samples isolated 12 strains, identified as the new Bunyan virus. Conclusion The epidemic of New Bunyan virus in the mountainous and hilly areas in the eastern part of our province is very similar to the hemorrhagic fever with renal syndrome. But in vitro cell culture and nucleotide sequence there is a clear difference.