目的：观察内源性激肽对培养新生大鼠心肌细胞生长的影响及其机制．方法：［３Ｈ］尿嘧啶和［３Ｈ］亮氨酸参入法检测ＲＮＡ和蛋白质合成速率，Ｎｏｒｔｈｅｒｎ杂交检测ｃｍｙｃ和ｃｆｏｓｍＲＮＡ表达．结果：卡托普利１００μｍｏｌ·Ｌ－１孵育４８ｈ显著抑制［３Ｈ］尿嘧啶和［３Ｈ］亮氨酸参入，孵育２ｈ明显抑制ｃｍｙｃ和ｃｆｏｓ基因表达．ＡｎｇⅡ１μｍｏｌ·Ｌ－１处理４８ｈ刺激ＲＮＡ和蛋白质合成，１ｈ可上调ｃｍｙｃ和ｃｆｏｓ表达．Ｃａｐ１００μｍｏｌ·Ｌ－１部分抑制ＡｎｇⅡ上述作用．缓激肽Ｂ２受体拮抗剂Ｉｃａ（０１－１０μｍｏｌ·Ｌ－１）剂量依赖性阻断Ｃａｐ作用．结论：内源性激肽经ＢＫＢ２受体介导对心肌细胞的生长起负调节作用 Objective: To observe the effect of endogenous kinin on the growth of cultured neonatal rat cardiomyocytes and its mechanism. Methods: [3H] uracil and [3H] leucine incorporation assay RNA and protein synthesis rate, Northern hybridization detection c  myc and c  fos mRNA expression. Results: After incubated with 100μmol·L-1 captopril for 48h, inhibition of [3H] uracil and [3H] leucine incorporation significantly inhibited the expression of c-myc and c-fos genes after incubated for 2h. Ang Ⅱ 1μmol·L-1 treatment 48h stimulate RNA and protein synthesis, 1h can be increased c  myc and c  fos expression. Cap100μmol·L-1 partially inhibits the above effects of AngⅡ. Bradykinin B2 receptor antagonist Ica (0.1-1μmol·L -1) dose-dependently blocked the action of Cap. CONCLUSION: Endogenous kinin is negatively regulated by BKB2 receptor on the growth of cardiomyocytes