论文部分内容阅读
以58份菊芋种质资源为试材,利用SRAP分子标记技术对其进行遗传多样性研究,为选育耐盐新品种提供参考依据。结果表明:从110对引物中筛选出16对多态性好的引物,共扩增出123条条带,其中多态性条带90条,多态性比率为72.7%。利用NTSYS软件统计分析出菊芋资源间遗传距离为0.01~0.52。应用UPGMA聚类可将58份菊芋资源划分为三大类群,第一大类群分为4个亚类群。表明利用SRAP技术更能充分揭示菊芋资源间的遗传差异,可作为种质鉴定依据。
58 accession germplasm resources of Jerusalem artichoke were used as materials to study their genetic diversity using SRAP molecular markers and to provide references for breeding new varieties with salt tolerance. The results showed that 16 pairs of primers were screened out from 110 pairs of primers. A total of 123 bands were amplified, of which 90 were polymorphic bands with a polymorphism rate of 72.7%. Using NTSYS software statistical analysis of Jerusalem artichoke resources between the genetic distance of 0.01 ~ 0.52. According to UPGMA clustering, 58 Jerusalem artichoke resources can be divided into three groups. The first group is divided into four subgroups. The results showed that using SRAP technology can fully reveal the genetic differences between Jerusalem artichoke resources, which can be used as germplasm identification basis.