建立血清中烟酸(NA)、烟酰胺(NM)及其代谢产物N′-甲基尼克酰胺(NMN)、2-吡啶酮(2Py)的测定方法。采用高效液相色谱法(HPLC),以氨基固相柱萃取样品,0.04mol/L KH2PO4提取洗脱,同时测定血清中NMN、NA、2Py、NM的含量。校准曲线线性范围为0.5—30μg/mL,r≥0.9986。检出限为NMN 0.45μg/mL,NA 0.51μg/mL,2Py 0.15μg/mL,NM 0.20μg/mL。回收率为88.6%—105.1%,相对标准偏差(RSD)为1.3%—7.8%。本法简单快速,准确,灵敏,达到了实验目的和要求。 To establish a method for determination of niacin (NA), nicotinamide (NM) and its metabolites N’-methylnicklamide (NMN) and 2-pyridone (2Py) in serum. High performance liquid chromatography (HPLC) was used to extract samples with amino solid phase column and eluted with 0.04mol / L KH2PO4. The contents of NMN, NA, 2Py and NM in serum were also determined. The calibration curve was linear over the range of 0.5-30 μg / mL with r ≥ 0.9986. The detection limits were NMN 0.45μg / mL, NA 0.51μg / mL, 2Py 0.15μg / mL, NM 0.20μg / mL. The recoveries were 88.6% -105.1% with relative standard deviations (RSDs) of 1.3% -7.8%. This method is simple, fast, accurate and sensitive, achieving the purpose and requirements of experiment.