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Gases in the marine boundary layer in Fildes Peninsula, maritime Antarctica were sampled and analyzed for the first time. Sampling sites included areas covered by moss and lichen growth, penguin colonies and an area where scientific research stations are located. A total of 211 samples were analyzed for N2O concentrations, with an average of (321.33±3.07) nL/L. This is above the global average value of 314 nL/L. It is found that the N2O concentrations evidently increase during the summer months. Concentrations around the research stations are higher than at remote areas, indicating a potential source from human activities on the island. N2O concentrations at a large penguin colony on Barton Peninsula are the highest among the sampled areas. This may explain why N2O concentrations in Fildes Peninsula are higher than the global average. Our preliminary conclusions are that human activities and emissions from penguin dropping-amended soil and vegetation soil constitute the major sources of N2O in the maritime Antarctic atmosphere.