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目的:建立蜂胶软胶囊中杨梅苷、芦丁、槲皮素、山柰酚、芹菜素、松属素、白杨素、高良姜素的HPLC测定方法。方法:Promosil C18柱;以甲醇(A)-0.1%磷酸溶液(B)为流动相进行梯度洗脱,流速:1.0 mL/min,柱温:30℃,检测波长:270 nm。结果:8种成分均能达到基线分离,各成分在其线性范围内呈良好线性关系,加样回收率在90%～110%。结论:该方法快速、准确、重复性好,可用于蜂胶软胶囊的含量测定。
OBJECTIVE: To establish a method for the determination of myricetin, rutin, quercetin, kaempferol, apigenin, procyanidins, chrysin, galangin in propolis soft capsules. Method: Promosil C18 column; gradient elution with methanol (A) -0.1% phosphoric acid solution (B) as mobile phase at a flow rate of 1.0 mL / min and a column temperature of 30 ℃ with a detection wavelength of 270 nm. Results: All the eight components could achieve the baseline separation. The linearity of each component showed a good linearity with the recoveries of 90% -110%. Conclusion: The method is rapid, accurate and reproducible. It can be used for the determination of propolis soft capsule.