论文部分内容阅读
目的 探讨nephrin在蛋白尿发生中的可能作用。方法 用免疫组化及图象分析的方法 ,分别对临床表现为大量蛋白尿 ,单纯性血尿患儿及对照组肾组织切片上nephrin的表达进行检测。结果 (1)大量蛋白尿组的nephrin表达量为 0 6 2± 0 2 4,与单纯性血尿组 (0 6 7± 0 2 3)及对照组(0 82± 0 17)比较三者之间差异无显著意义 (P >0 0 5 )。 (2 )大量蛋白尿伴弥漫性足突融合组nephrin表达量为 0 6 1± 0 2 5 ,与大量蛋白尿不伴弥漫足突融合组 (0 6 2± 0 2 5 )及对照组比较三者间差异无显著意义 (P >0 0 5 )。 (3)弥漫足突融合微小病变组nephrin表达量为 0 5 0± 0 15 ,与非微小病变 (0 6 5±0 2 7)及对照组比较差异无显著意义 (P >0 0 5 )。结论 在获得性肾小球疾病中 ,用免疫组化的方法未能检测到肾小球nephrin的表达变化。
Objective To investigate the possible role of nephrin in the development of proteinuria. Methods Immunohistochemical and image analysis methods were used to detect the expression of nephrin on renal biopsy specimens of patients with massive proteinuria, simple hematuria and control group respectively. Results (1) The expression of nephrin in mass albuminuria group was 0 6 2 ± 0 2 4, which was significantly higher than that in simple hematuria group (0 6 7 ± 0 2 3) and control group (0 82 ± 0 17) There was no significant difference (P> 0.05). (2) The expression of nephrin in mass proteinuria with diffuse foot process was 0 6 1 ± 0 25, compared with that of large proteinuria without diffuse foot process (0 6 2 ± 0 2 5) and control group There was no significant difference between the two groups (P> 0.05). (3) The expression level of nephrin in diffuse mininal protrusion fusion group was 0 50 ± 0 15, which had no significant difference with non-minimal change (0 65 ± 0 27) and control group (P 0 05). Conclusions In acquired glomerular diseases, the expression of glomerular nephrin can not be detected by immunohistochemistry.